jGCaMP8 mouse ground truth dataset

This dataset contains synchronized two‑photon calcium‑imaging movies and loose‑patch cell‑attachment recordings from layer‑2 pyramidal cells and interneurons in the primary visual cortex of wild‑type mice. Recordings were performed in vivo while presenting moving grating stimuli to the contralateral eye. Indicators (jGCaMP8f/m/s, jGCaMP7f, XCaMPgf) were expressed via AAV injection under the synapsin‑1 promoter. Multiple neurons were recorded per mouse, and each cell was recorded multiple times in ~3‑minute sessions (movie & scan). A single .nwb file aggregates all data for a given cell. The dataset includes motion‑corrected two‑photon movies, raw loose‑patch electrophysiology traces for individual neurons, cell bodies and neuropil ROIs segmented by Suite2p, fluorescence traces for all ROIs, and detailed information about the moving grating stimulus presented to the contralateral eye.

Updated 12/3/2022

github

Description

jGCaMP8 Mouse Ground Truth Dataset Summary

Dataset Description

Content

Simultaneous 2-photon calcium imaging movies and loose-seal cell attached recordings of upper layer 2 pyramidal cells and interneurons in the primary visual cortex of wild type mouse, in vivo, during drifting gratings stimulus in the contralateral eye.
Multiple neuron recordings per mouse, with multiple ~3-minute-long recordings (movies & sweeps) per cell.
One .nwb file per cell, containing all data.

Features

Motion corrected 2-photon movies
Raw cell attached loose-seal electrophysiology traces of a single neuron in the imaging field of view
Somatic cellular and neuropil ROIs segmented with Suite2p
Fluorescence traces for all ROIs
Details of the drifting gratings stimuli presented to the contralateral eye

Expression

jGCaMP8f, jGCaMP8m, jGCaMP8s, jGCaMP7f, and XCaMPgf expressed via AAV injections using synapsin-1 promoter.

Data Access

Download Options:
- Stream data from DANDI archives
- Download whole dataset (1.4 TB)
- Download data of a single cell
Command for Local Download:

dandi download DANDI:000168

Dataset Location

DANDIset: https://dandiarchive.org/dandiset/000168/

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Topics

Neuroscience

Calcium Imaging

Source

Organization: github

Created: 4/30/2022

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