jGCaMP8 mouse ground truth dataset
This dataset contains synchronized two‑photon calcium‑imaging movies and loose‑patch cell‑attachment recordings from layer‑2 pyramidal cells and interneurons in the primary visual cortex of wild‑type mice. Recordings were performed in vivo while presenting moving grating stimuli to the contralateral eye. Indicators (jGCaMP8f/m/s, jGCaMP7f, XCaMPgf) were expressed via AAV injection under the synapsin‑1 promoter. Multiple neurons were recorded per mouse, and each cell was recorded multiple times in ~3‑minute sessions (movie & scan). A single .nwb file aggregates all data for a given cell. The dataset includes motion‑corrected two‑photon movies, raw loose‑patch electrophysiology traces for individual neurons, cell bodies and neuropil ROIs segmented by Suite2p, fluorescence traces for all ROIs, and detailed information about the moving grating stimulus presented to the contralateral eye.
Dataset description and usage context
jGCaMP8 Mouse Ground Truth Dataset Summary
Dataset Description
Content
- Simultaneous 2-photon calcium imaging movies and loose-seal cell attached recordings of upper layer 2 pyramidal cells and interneurons in the primary visual cortex of wild type mouse, in vivo, during drifting gratings stimulus in the contralateral eye.
- Multiple neuron recordings per mouse, with multiple ~3-minute-long recordings (movies & sweeps) per cell.
- One .nwb file per cell, containing all data.
Features
- Motion corrected 2-photon movies
- Raw cell attached loose-seal electrophysiology traces of a single neuron in the imaging field of view
- Somatic cellular and neuropil ROIs segmented with Suite2p
- Fluorescence traces for all ROIs
- Details of the drifting gratings stimuli presented to the contralateral eye
Expression
- jGCaMP8f, jGCaMP8m, jGCaMP8s, jGCaMP7f, and XCaMPgf expressed via AAV injections using synapsin-1 promoter.
Data Access
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Download Options:
- Stream data from DANDI archives
- Download whole dataset (1.4 TB)
- Download data of a single cell
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Command for Local Download:
dandi download DANDI:000168
Dataset Location
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